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Next Generation Sequencing Facilitates Quantitative Analysis of uterine NK cell transcriptomes from Pbx1f/f and Pbx1f/f; Ncr1Cre mice

GSE125944 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/12/31 Platform GPL21273
Summary
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to investigatethe effect of the Pbx1 gene on the gene expression program of mature uterine NK cells. Methods: The differential analysis of gene expression profiles in uterine NK cells from Pbx1f/f and Pbx1f/f; Ncr1Cre mice at gd11.5 by RNA-Seq . Results: Using an optimized data analysis workflow, we found that the inactivation of PBX1 altered the NK cell gene expression program, and many gene expression changes, including significant down-regulation of Ptn and Ogn expression. Conclusions: PBX1 not only regulates the proliferative capacity of NK cells but also promotes the expression of GPFs in NK cells.
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Direct links to NCBI, no account and no request form: the whole study as GSE125944_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA518049 and SRA study SRP183029. Searching any of these in the dataset finder brings you back here.

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