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A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice

GSE12757 Mus musculus Non-coding RNA profiling by high throughput sequencing 9 samples Submitted 2008/10/01 Platform GPL9185
Summary
We examined the construction of the piRNA system in the restricted developmental window in which methylation patterns are set during mammalian embryogenesis. We find robust expression of two Piwi family proteins, MIWI2 and MILI. Their associated piRNA profiles reveal differences from Drosophila wherein large piRNA clusters act as master regulators of silencing. Instead, in mammals, dispersed transposon copies initiate the pathway, producing primary piRNAs, which predominantly join MILI in the cytoplasm. MIWI2, whose nuclear localization and association with piRNAs depend upon MILI, is enriched for secondary piRNAs antisense to the elements that it controls. The Piwi pathway lies upstream of known mediators of DNA methylation, since piRNAs are still produced in Dnmt3L mutants, which fail to methylate transposons. This implicates piRNAs as specificity determinants of DNA methylation in germ cells.
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Also filed as BioProject PRJNA111011 and SRA study SRP000623. Searching any of these in the dataset finder brings you back here.

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