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mRNA-Seq Whole Transcriptome Analysis of a Single Cell

GSE14605 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2009/02/23 Platform GPL9318
Summary
Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a single cell whole transcriptome assay. The modified amplification strategy allows us to amplify full-length cDNAs for most of the expressed genes. We show that it is feasible to get digital gene expression profiles at single cell resolution. This allows us to ask fundamental biological questions that could not be addressed previously, especially in the early embryonic development field, and to understand transcriptome complexity at the resolution of a single cell.
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Also filed as BioProject PRJNA111725. Searching any of these in the dataset finder brings you back here.

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