Gene expression profile at single cell level of whole lungs of mice collected from bleomycin or PBS-treated young and old mice at day 14, day 28 and day 60 post injection
Single-cell RNA-seq · mouse · 12 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Aging affects reprogramming of pulmonary capillary endothelial cells after lung injury in male miceNature communications · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE234197_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA980275 and SRA study SRP441200. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7454030 Young D14, scRNAseq
- GSM7454031 Young D14, HTO
- GSM7454032 Old D14, scRNAseq
- GSM7454033 Old D14, HTO
- GSM7454034 Young D28, scRNAseq
- GSM7454035 Young D28, HTO
- GSM7454036 Old D28, scRNAseq
- GSM7454037 Old D28, HTO
- GSM7454038 Young D60, scRNAseq
- GSM7454039 Young D60, HTO
- GSM7454040 Old D60, scRNAseq
- GSM7454041 Old D60, HTO
- GSE234198 Spatial RNA sequencing of bleomycin-treated young and old mice lungs at day 14 and day 28 post injection 8 samples shares bleomycin, injection, lungs, young
- GSE290920 Single cell RNAseq of whole lungs extracted from Bleomycin-induced pulmonary fibrosis models of wild type and Kdm7a KO mice. 2 samples shares bleomycin, whole, lungs
- GSE262965 Investigation of CD64+ macrophages in bleomycin treated lungs [NGS2117] 24 samples shares bleomycin, lungs
- GSE325299 Sex difference in mRNA expressions of lungs post bleomycin-induced acute lung injury 20 samples shares bleomycin, lungs
- GSE281154 mRNA transcriptome sequencing of tumor cells, TRMs, MDMs, Monocytes and CD8+ T cells from tumor lungs of KrasLSL-G12D/+Lkb1fl/fl (KL), KrasLSL-G12D/+Lkb1fl/flGpx4m/m(KLG4) after Ad-cre injection and tamoxifen treatment 17 samples shares injection, lungs
- GSE262926 Micro bulk sequencing on murine Bleomycin-induced fibrotic lungs sorted on SA-β-Gal and GD3 15 samples shares bleomycin, lungs
- GSE274184 Transcriptomic study on the effect of Igf1r deficiency in control and bleomycin-challenged mouse lungs. 12 samples shares bleomycin, lungs
- GSE307366 RNA-seq of lungs from SCID mice pre-treated with EVs from Tsc1-null or wild-type E15.5 neural progenitors prior to ELT3 cell injection 6 samples shares injection, lungs
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.