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The RNA-binding protein PTBP3 regulates alternative polyadenylation in naïve B cells

GSE148899 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/04/16 Platform GPL17021Platform GPL21273
Summary
As an important family of RNA-binding proteins, PTB family plays crucial roles in post-transcriptional regulation of gene expression. PTBP1 and PTBP2 have been extensively characterized as important splicing factors regulating many biological processes. PTBP3 is reported to be essential for AID-targeting specificity in activated B cells. However, the physiological roles and regulatory mechanisms of PTBP3 in naive B cells are largely unknown. Here, we identified PTBP3 as a regulator of alternative polyadenylation using 2P-seq data in naive B cells. We revealed that PTBP3 promoted poly(A) signal recognition through binding to CU-rich elements in 3’ UTR and interacting with CPSF6 factor in a position- and dosage-dependent manner. Furthermore, we also demonstrated that PTBP3 could regulate mRNA stability by facilitating the usage of different poly(A) sites in mRNA. Our study will provide novel insights into the PTBP3 functions in innate immunity and immunological diseases.
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Direct links to NCBI, no account and no request form: the whole study as GSE148899_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA626431 and SRA study SRP257346. Searching any of these in the dataset finder brings you back here.

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