GEO series

Highly Efficient generation of Human Hepatic Cells from Induced Pluripotent Stem Cells.

GSE14897 Homo sapiens (human) Expression microarray 15 samples Submitted 2009/08/01 Platform GPL570

At a glance

Expression microarray · human · 15 samples

What it is: Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism: Homo sapiens (human)
Samples: 15 samples
Data you can get: A processed expression table (series matrix) and the array files, from NCBI.

Summary (written by the submitters)

Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages.

Read the full summary ↓

We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.

This dataset

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Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗

3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE14897_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA111995. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 19998274) ↗

Samples in this study

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+ 15 more — browse all 15 samples with per-sample file links →

Datasets on a similar topic

GSE249826 Human-induced pluripotent Stem Cell-Derived Hepatic Stellate Cells in Liver Organoids: Maturation Promotion and Essential Role in Vascularization 26 samples shares pluripotent, hepatic, induced, stem
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GSE293032 Transcriptomic comparison of hepatocytes derived from a panel of human induced pluripotent stem cells with different ethnicities 54 samples shares pluripotent, induced, stem
GSE267656 Efficient generation of human retinal pigment epithelium cells via chemically induced pluripotency 39 samples shares generation, efficient, induced
GSE269571 Generation of Stable Chambered Cardioids from human Pluripotent Stem Cells [bulk RNA-seq] 24 samples shares pluripotent, generation, stem

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