← BioTransfer GEO Dataset Finder
GEO series

Molecular characterization of endosomal self RNA Rmrp-engaged TLR3 dimerization to prime innate activation

GSE159459 Mus musculus Expression profiling by high throughput sequencing; Other 4 samples Submitted 2025/04/01 Platform GPL17021Platform GPL21273
Summary
In this study, we demonstrate that endosome-localized self RNA Rmrp directly binds to TLR3 and induces TLR3 dimerization in the early endosome but does not interact with endosome-localized TLR7, TLR8, TLR9 or cytoplasmic RNA sensor RIG-I under homeostatic conditions. Cryo-EM structure of Rmrp-TLR3 complex reveals a novel lapped conformation of TLR3 dimer engaged by Rmrp, which is distinct from the activation mechanism by dsRNA and the specific structural feature at the 3'-end of Rmrp is critical for its functional interaction with TLR3. Furthermore, K42 residue of TLR3 is essential for binding to Rmrp and subsequent dimerization. Rmrp dissociates from TLR3 following endosomal acidification, generating a matured TLR3 dimer which is primed for innate recognition and activation. Myeloid-cell deficiency of Rmrp reduces TLR3 dimerization and attenuates TLR3-mediated antiviral responses against influenza A both in vitro and in vivo.
Published in
Molecular characterization of endosomal self RNA Rmrp-engaged TLR3 dimerization to prime innate activation
Zhang S, Li B, Liu L et al. · Cell research 2025 · PMID 40915996 · doi:10.1038/s41422-025-01178-5
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE159459_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA669002 and SRA study SRP287261. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.