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Next Generation Sequencing of Transcriptomes from Murine Articular Chondrocytes Treated with TGFb1 and BMP2 [RNA-seq]

GSE169383 Mus musculus Expression profiling by high throughput sequencing 7 samples Submitted 2026/03/25 Platform GPL21103
Summary
Murine articular chondrocytes were isolated from femoral heads of 3-week-old wild-type C57BL/6J pups as described (Gosset et al., 2008) with modifications. Following digestion, chondrocytes were harvested and cultured in complete high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco; #31053028) supplemented with 10% fetal bovine serum (FBS; Gibco; #10437), 2 mM L-Glutamine (Gibco; #A2916801), and 1% penicillin/streptomycin. Primary chondrocytes were seeded at a density of 50 x 104 cells/well in 12-well plates and treated with vehicle, 5 ng/ml TGFβ1 (R&D Systems; #240-B-010) and 100ng/ml BMP2 (R&D Systems; #355-BM-010) for 24 hours. Total RNA was then extracted from cell cultures and performed RNA sequencing.
Published in
NFIA regulates articular chondrocyte fatty acid metabolism and joint homeostasis
Wang C, Fang L, Shi M et al. · Science translational medicine 2025 · PMID 40737429 · doi:10.1126/scitranslmed.adm9488
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Direct links to NCBI, no account and no request form: the whole study as GSE169383_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA716351 and SRA study SRP311705. Searching any of these in the dataset finder brings you back here.

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