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Loss of FBXO11 establishes a stem cell program in acute myeloid leukemia by dysregulating LONP1

GSE183844 Homo sapiens (human) Expression microarray 32 samples Submitted 2025/10/21 Platform GPL20795Platform GPL17586
At a glance

Expression microarray · human · 32 samples

What it is
Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism
Homo sapiens (human)
Topic
Acute myeloid leukemiaThis series is part of our curated briefing on it.
Samples
32 samples: 11 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed expression table (series matrix) and the array files, from NCBI.
Paper
Loss of FBXO11 establishes a stem cell program in acute myeloid leukemia by dysregulating LONP1The Journal of clinical investigation · 2026
Summary (written by the submitters)
Acute myeloid leukemia (AML) is an aggressive cancer with very poor outcomes. To identify additional drivers of leukemogenesis, we analyzed sequencing data from 1,727 unique individual patients with AML, which revealed mutations in ubiquitin ligase family genes in 11.2% of samples from adult patients with AML with mutual exclusivity. The SKP1/CUL1/F-box (SCF) E3 ubiquitin ligase complex gene, FBXO11, was the most significantly downregulated gene of the SCF complex in AML.
Read the full summary ↓
We found that FBXO11 interacts with and catalyzes K63-linked ubiquitination of LONP1 in the cytosol, to promote LONP1 entry into mitochondria. We show that depletion of FBXO11 or LONP1 reduced mitochondrial respiration through impaired LONP1 chaperone activity to assemble electron transport chain Complex IV. Reduced mitochondrial respiration secondary to FBXO11 or LONP1 depletion imparted myeloid-biased stem cell properties in primary CD34+ hematopoietic stem and progenitor cells (HSPCs) in vitro. In a human xenograft model, depletion of FBXO11 cooperated with AML1-ETO and mutant KRASG12D to generate serially transplantable AML. Our findings suggest that reduced FBXO11 cooperates to initiate AML by priming HSPC for myeloid-biased self renewal through attenuation of LONP1-mediated regulation of mitochondrial respiration.
Published in
Loss of FBXO11 establishes a stem cell program in acute myeloid leukemia by dysregulating LONP1
Kincross H, Mo YA, Wang X et al. · The Journal of clinical investigation 2026 · PMID 41289019 · doi:10.1172/JCI181943
This dataset
Open GSE183844 in finder → Find more human microarray datasets → Acute myeloid leukemia landscape →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE183844_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA762075 and SRA study SRP336419. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41289019) ↗
Study design
11 conditions, mostly in triplicate
shCT ×3 shFBXO11_9 ×3 shLONP1_1 ×3 Empty ×3 FBXO11 ×3 LONP1 ×3 shLONP1_FBXO11 ×3 CB15p-shFBXO11 ×2 +6 more

Supports a between-group comparison across 29 samples.

How we read this: 11 replicated groups read from 32 sample titles; they account for 29 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
More Acute myeloid leukemia datasets

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