GEO series

AMTB, a TRPM8 Antagonist, Suppresses Growth and Metastasis of Osteosarcoma through Repressing the TGFβ Signaling Pathway

GSE188304 Homo sapiens (human) RNA-seq (gene expression) 2 samples Submitted 2024/11/01 Platform GPL11154

At a glance

RNA-seq (gene expression) · human · 2 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Topic: OsteosarcomaThis series is part of our curated briefing on it.
Samples: 2 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Since its first identification in prostate cancers and prostate tissues, transient receptor potential melastatin-subfamily member 8 (TRPM8) is subsequently found to be overexpressed in a wide range of cancers and is shown to be implicated in tumorigenesis and tumor progression. Here, we used N-(3-aminopropyl)-2-[(3-methylphenyl) methoxy] -N-(2-thienylmethyl) benzamide hydrochloride (AMTB), a specific TRPM8 antagonist, to explore its antitumoral effect on osteosarcoma.

Read the full summary ↓

We find that AMTB suppress osteosarcoma cell proliferation, metastasis and induce cellular apoptosis. Xenograft model in nude mice experiments also define that AMTB can increase the sensitivity of tumor cells to cisplatin, the cytotoxic chemotherapeutic regimens in treating osteosarcoma. Molecularly, AMTB specific antagonizes TRPM8 which is upregulated in osteosarcoma and its expression level in osteosarcoma tissues is negatively related to patients’ prognosis. Finally, RNA sequencing analysis was performed to explore the mechanism underlying the antitumoral effect of AMTB on osteosarcoma cells and the results prove that AMTB suppresses the Transforming Growth Factor β (TGFβ) signaling pathway. Our study provides evidence that TRPM8 could be a potential therapeutic target and AMTB can suppress growth and metastasis of osteosarcoma cells through repressing the TGFβ signaling pathway and increase the sensitivity of tumor cells to cisplatin.

This dataset

Open GSE188304 in finder → Find more human RNA-seq datasets → Osteosarcoma landscape →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE188304_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA778237 and SRA study SRP344799. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

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+ 2 more — browse all 2 samples with per-sample file links →

More Osteosarcoma datasets

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GSE250015 Exploring the impact of PDGFD in osteosarcoma metastasis through single-cell sequencing analysis 2 samples same disease briefing · shares osteosarcoma, metastasis
GSE259286 In vitro modeling of nutritional and mitochondrial-targeted therapies for osteosarcoma 98 samples same disease briefing · shares osteosarcoma
GSE253548 The whole transcriptome analysis using FFPE and fresh tissue samples identifies the molecular fingerprint of osteosarcoma 90 samples same disease briefing · shares osteosarcoma
GSE152048 Single cell analysis of osteosarcoma tissues 76 samples same disease briefing · shares osteosarcoma
GSE317512 Age-stratified transcriptomic profiling reveals biologically distinct molecular phenotypes across pediatric, adolescent, and adult osteosarcoma 70 samples same disease briefing · shares osteosarcoma
GSE218035 Long-read transcriptome sequencing reveals expression characteristics of osteosarcoma 36 samples same disease briefing · shares osteosarcoma

Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.