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Using VBIM technique to discover ARMC4/ODAD2 as a novel negative regulator of NF-κB and a new tumor suppressor in colorectal cancer

GSE197313 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/01/31 Platform GPL24676
Summary
Since nuclear factor (NF) κB plays pivotal roles in inflammation and cancer, understanding its regulation holds great promise for disease therapy. Using the powerful validation-based inser-tional mutagenesis (VBIM) technique established by us previously, we discovered armadillo re-peat containing protein 4 (ARMC4), a rarely studied protein known to date, as a novel negative regulator of NF-κB in colorectal cancer (CRC). High expression of ARMC4 downregulated the expression of NF-κB-dependent genes. We report the expression differences of NF-kB target genes in a novel inhibitor of NF-kB activity. Through RNA-seq we identified a group of NF-kB target genes specifically regulated by ARMC4. This study provides a framework for identifying genes downregulated and upregulated by a novel inhibitor of NF-kB activity.
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Also filed as BioProject PRJNA809737 and SRA study SRP361180. Searching any of these in the dataset finder brings you back here.

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