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RNA Sequencing Facilitates Quantitative Analysis of Lupus (MRL/lpr) and Control(MRL/mpj)Mouse Microglia Tanscriptomes

GSE201282 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/03/27 Platform GPL24247
Summary
Up to 75% of systematic lupus erythematosus (SLE) patients experience neuropsychiatric (NP) symptoms, called neuropsychiatric SLE (NPSLE), yet the underlying mechanisms remain elusive. Microglia control synaptic pruning during early postnatal brain development. The process in NPSLE remains unclear. Here, we show that microglia-coordinated elimination of synaptic terminals participated in NPSLE in MRL/lpr mice, a lupus-prone murine model. We elucidated that lupus mice developed increased depression- and anxiety-like behaviors and persistent phagocytic microglia reactivation before overt peripheral lupus pathology. Microglial engulfment of synapses explained behavioral disorders. To elucidate the mechanism of synaptic pruning by microglia, we sequenced the gene expression in sorted microglia from both lupus (MRL/lpr) mice and the wild-type (MRL/mpj) controls.
Published in
Microglia orchestrate synaptic and neuronal stripping: Implication in neuropsychiatric lupus
Zhou Y, Chen L, Zheng X et al. · Journal of cellular and molecular medicine 2024 · PMID 38494844 · doi:10.1111/jcmm.18190
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Also filed as BioProject PRJNA830717 and SRA study SRP371690. Searching any of these in the dataset finder brings you back here.

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