Effect of BTK and GSK3 inhibition on gene expression in primary peripheral human B cells
RNA-seq (gene expression) · human · 15 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 15 samples: 15 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Oxidative phosphorylation regulates B cell effector cytokines and promotes inflammation in multiple sclerosisScience immunology · 2024
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE206050_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA848850 and SRA study SRP380084. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 15 sample titles: 15 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM6241001 Donor 1, B cells, Vehicle, Nil
- GSM6241002 Donor 2, B cells, Vehicle, Nil
- GSM6241003 Donor 3, B cells, Vehicle, Nil
- GSM6241004 Donor 1, B cells, BTKi, Nil
- GSM6241005 Donor 2, B cells, BTKi, Nil
- GSM6241006 Donor 3, B cells, BTKi, Nil
- GSM6241007 Donor 1, B cells, GSK3i, Nil
- GSM6241008 Donor 2, B cells, GSK3i, Nil
- GSM6241009 Donor 3, B cells, GSK3i, Nil
- GSM6241010 Donor 1, B cells, Vehicle, anti-BCR
- GSM6241011 Donor 2, B cells, Vehicle, anti-BCR
- GSM6241012 Donor 3, B cells, Vehicle, anti-BCR
- GSM6241013 Donor 1, B cells, BTKi, anti-BCR
- GSM6241014 Donor 2, B cells, BTKi, anti-BCR
- GSM6241015 Donor 3, B cells, BTKi, anti-BCR
- GSE341102 Single-cell sequencing reveals the immune cell landscape of peripheral blood mononuclear cells in primary membranous nephropathy 8 samples shares peripheral, primary
- GSE300177 Catalytic inhibition of KAT6/KAT7 enhances the efficacy and overcomes primary and acquired resistance to Menin inhibitors in MLL leukaemia 194 samples shares inhibition, primary
- GSE330399 Molecular and Structural Basis of Pan-Resistance to BTK Inhibitors and Degraders 100 samples shares btk
- GSE243841 Peripheral mRNA expression of first-episode drug-naïve patients with schizophrenia 181 samples shares peripheral
- GSE327707 Sorted bulk RNA-seq profiling of human peripheral blood mononuclear cell populations across diverse in vitro immune stimuli 150 samples shares peripheral
- GSE310095 Whole Blood Transcriptomics Differentiates Circulating Gene Expression Between Coronary Artery Disease and Peripheral Artery Disease 71 samples shares peripheral
- GSE288704 Whole transcriptome analysis of unmutated sporadic ALS patients’ peripheral blood reveals phenotype-specific gene expression signature 66 samples shares peripheral
- GSE298581 Global gene expression response to PPARG inhibition in three urothelial cancer cell lines 48 samples shares inhibition
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.