GEO series

Multipotent neural stem cells originating from neuroepithelium exist outside of the mouse central nervous system [scRNA-seq]

GSE213132 Mus musculus (mouse) Single-cell RNA-seq 3 samples Submitted 2025/01/10 Platform GPL19057

At a glance

Single-cell RNA-seq · mouse · 3 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 3 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Multipotent neural stem cells originating from neuroepithelium exist outside the mouse central nervous systemNature cell biology · 2025

Summary (written by the submitters)

It has been widely accepted that mammalian neural stem cells (NSCs) exist only in the central nervous system. Here, we report that peripheral NSCs (pNSCs) exist outside of the CNS and can be isolated—from mouse embryonic limb, postnatal lung, tail, dorsal root ganglia, and adult lung tissues. Derived pNSCs are distinguished from neural crest stem cells, express multiple NSC-specific markers and exhibit cell morphology, self-renewing capacity, genome-wide transcriptional profile, epigenetic features, and differentiation ability similar to those of control brain NSCs.

Read the full summary ↓

pNSCs are from Sox1+ cells and originate from neuroepithelial cells. Single-cell RNA sequencing identifies that pNSCs in situ show similar molecular feature to brain NSCs. Furthermore, many pNSCs that migrate out of the neural tube can differentiate into neurons and limited glia cells during embryo development. Our finding of the existence of pNSCs provides previously unidentified insight of the mammalian nervous system development and presents an alternative potential strategy for neural regenerative therapy.

Published in

Multipotent neural stem cells originating from neuroepithelium exist outside the mouse central nervous system

Han D, Xu W, Jeong HW et al. · Nature cell biology 2025 · PMID 40211073 · doi:10.1038/s41556-025-01641-w

This dataset

Open GSE213132 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE213132_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA879225 and SRA study SRP396645. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40211073) ↗

Samples in this study

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+ 3 more — browse all 3 samples with per-sample file links →

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