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RNAseq of low-pigmented and high-pigmented cells from mouse B16 melanoma cell lines (experiment 1)

GSE214826 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/07/09 Platform GPL19057
Summary
Functional variation due to underlying genetic and phenotypic heterogeneity poses a major challenge to long-term melanoma management. Melanin pigment production is the hallmark of normal melanocytic differentiation but varies significantly between and within melanoma tumours. Detailed assessment of the functional consequences of pigment heterogeneity in viable melanoma samples has been experimentally challenging. Here, we developed a novel method to prospectively separate melanoma cell populations based on the presence of intracellular melanin using fluorescence-activated cell sorting (FACS). We find that nearly all pigmented patient melanomas and melanoma cell lines comprise mixtures of cells ranging from very high to low or undetectable pigment content. Using viable sorted cells, we interrogated the functional characteristics of low pigmented cells (LPC) and high pigmented cells (HPC) in the B16-F10 mouse melanoma cell line.
Published in
Prospective Isolation According to Melanin Pigment Content of Melanoma Cells With Heterogeneous Potentials for Disease Propagation
Fedele C, Kuser-Abali G, Rossi R et al. · Pigment cell & melanoma research 2025 · PMID 40588721 · doi:10.1111/pcmr.70011
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Also filed as BioProject PRJNA887336 and SRA study SRP401076. Searching any of these in the dataset finder brings you back here.

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