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Mitochondrial fission mediates an evolutionarily conserved innate defense response against bacterial infection

GSE225734 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 3 samples Submitted 2025/11/30 Platform GPL19057
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 3 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
3 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Animals must be able to engage pleiotropic immune defense mechanisms against microorganisms to survive infection. Here we reveal an unexpected function for mitochondrial fission in innate immunity, showing this process mediates host defense through antimicrobial lipid droplet production. Bacterial infection activates fission, enabling microbial clearance in mammalian macrophages and Caenorhabditis elegans.
Read the full summary ↓
The mechanism involves the mitochondrial unfolded protein response (UPRmt), characterized by activation of Atf transcription factor family members in mouse (Atf5) and C. elegans (ATFS-1). Fission and the UPRmt initiate bacterial killing through inducible production of lipid droplets, organelles with antimicrobial properties. Salmonella enterica serovar Typhimurium blocks inducible fission but inhibiting the fusion-promoting enzyme histone deacetylase 6 (Hdac6) overcomes this host subversion, enabling bacterial clearance. We propose that mitochondrial fission has similarities to intracellular bacterial replication, forewarning the host of impending infection. Awakening this ancient pathway in bacterial pathogen-compromised cells could be exploited for host-directed anti-infective strategies.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE225734_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA937243 and SRA study SRP423743. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 3 more — browse all 3 samples with per-sample file links →

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