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Chronic stress disrupts hepatic homeostasis and accelerates liver cancer progression through ADRB2 signaling

GSE228579 Mus musculus (mouse) Single-nucleus RNA-seq 66 samples Submitted 2026/05/01 Platform GPL21103
At a glance

Single-nucleus RNA-seq · mouse · 66 samples

What it is
Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue). Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Mus musculus (mouse)
Samples
66 samples: 16 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Chronic stress disrupts hepatic homeostasis and accelerates liver cancer progression through ADRB2 signalingScience advances · 2026
Summary (written by the submitters)
Chronic stress has been implicated in the dysregulation of immunological, neurochemical, and endocrinological functions, yet its impact on hepatic homeostasis and liver carcinogenesis remains elusive. In this study, by employing single-nucleus RNA sequencing and histopathological evaluation, we demonstrated that chronic stress induced profound hepatic dysfunction.
Read the full summary ↓
Strikingly, using orthotopic murine models of liver cancer, we further found that chronic stress significantly accelerated tumor progression. Mechanistically, we identified β2-adrenergic receptor (ADRB2) signaling as the pivotal molecular pathway driving stress-accelerated cancer progression, which was in line with the poor clinical outcomes observed in cancer patients exhibiting enhanced adrenergic signaling within tumors. Collectively, our study provides compelling evidence that chronic stress compromises hepatic homeostasis and accelerates liver cancer progression via ADRB2 activation, highlighting the therapeutic potential of targeting this pathway and the clinical importance of stress management in hepatic disorders.
Published in
Chronic stress disrupts hepatic homeostasis and accelerates liver cancer progression through ADRB2 signaling
Qin H, Chu L, Zheng X et al. · Science advances 2026 · PMID 42066073 · doi:10.1126/sciadv.aec0825
This dataset
Open GSE228579 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE228579_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA950389 and SRA study SRP430155. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42066073) ↗
Study design
16 conditions, mostly with about 4 replicates each
Liver, social defeat stress, ×6 Liver, restraint stress, ×5 Liver, without stress, ×4 Liver, foot shock stress, ×4 Liver, liver cancer, ×4 Liver, stressed liver cancer, ×4 Adrb2-control hepatocytes, ×4 Adrb2-deficient hepatocytes, ×4 +14 more

Supports a case/control comparison: 17 samples read as cases, 8 as controls.

How we read this: 16 replicated groups read from 66 sample titles; they account for 60 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 26 more — browse all 66 samples with per-sample file links →

Datasets on a similar topic

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