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yHSC AThi oHSC ATlo oHSC Bulk RNA-seq - Understanding transcriptional changes between autophagy engaging and non-engaging hematopoietic stem cells during mouse aging.

GSE230279 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/05/15 Platform GPL21103
Summary
We sought to understand the transcriptional profile of autophagy engaging vs non-engaging old hematopoietic stem cells (HSC) referenced against young HSCs. Bulk RNA-seq analyses indicated a large transcriptional divergence between the two oHSC subsets, with pathway analyses of differentially expressed genes (DEG) demonstrating enrichment in inflammatory signaling in AThi oHSCs and in oxidative metabolism signaling in ATlo oHSCs. Ingenuity Pathway Analysis (IPA) inferred that several inflammation-coupled pro-autophagy regulators including p53, Bnip3l, Nupr1, and Nlrp3[22,23], in addition to quiescence enforcing checkpoints FoxO3a, Rb1 and Cdkn1a, were activated in AThi oHSCs.
Published in
Autophagy counters inflammation-driven glycolytic impairment in aging hematopoietic stem cells
Dellorusso PV, Proven MA, Calero-Nieto FJ et al. · Cell stem cell 2024 · PMID 38754428 · doi:10.1016/j.stem.2024.04.020
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Also filed as BioProject PRJNA958181 and SRA study SRP433778. Searching any of these in the dataset finder brings you back here.

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