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yHSC and oHSC 10X scRNAseq under ad lib, fasted, or fast refeed conditions to evaluate transcriptional impact of transient autophagy engagement in hematopoietic stem cells.

GSE230284 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/05/15 Platform GPL24247
Summary
We observed that 24hr of fasting followed by a 24hr refeeding period improved the regenerative capacity of hematopoietic stem cells isolated from old mice, which was associated with an increase in glycolytic capacity. We sought to evaluate transcriptional changes in yHSC and oHSC compartment following different mouse feeding paradigms at single cell resolution. In yHSCs, AL and F/R groups showed overlapping transcriptional signatures with a distinct pattern for the fasted group. In contrast, in oHSCs, AL and fasted groups showed a strong overlap with a distinct transcriptional signature for the F/R group, reflective of differences in metabolic set points with age.
Published in
Autophagy counters inflammation-driven glycolytic impairment in aging hematopoietic stem cells
Dellorusso PV, Proven MA, Calero-Nieto FJ et al. · Cell stem cell 2024 · PMID 38754428 · doi:10.1016/j.stem.2024.04.020
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Also filed as BioProject PRJNA958196. Searching any of these in the dataset finder brings you back here.

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