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Systematic identification of Y-chromosome gene functions in mouse spermatogenesis [10x]

GSE274095 Mus musculus (mouse) Single-nucleus RNA-seq 16 samples Submitted 2025/01/24 Platform GPL21103Platform GPL24247
At a glance

Single-nucleus RNA-seq · mouse · 16 samples

What it is
Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue). Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Mus musculus (mouse)
Samples
16 samples: 5 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Systematic identification of Y-chromosome gene functions in mouse spermatogenesisScience (New York, N.Y.) · 2025
Summary (written by the submitters)
The mammalian Y chromosome is essential for male fertility, but how individual Y genes regulate spermatogenesis is poorly understood. To resolve this question, we generate a deletion series of the mouse Y chromosome, creating thirteen Y-deletant mouse models and conducting exhaustive reproductive phenotyping. Eight Y genes, including several that are deeply conserved and exhibit testis-specific expression, are dispensable for spermatogenesis.
Read the full summary ↓
For others, we uncover novel functions, including a role for Uty in establishment and differentiation of spermatogonia, and for Zfy2 in ensuring meiotic pairing and reciprocal recombination between the sex chromosomes. We also generate the first mouse equivalent of the human infertility AZFa deletion, revealing cumulative detrimental effects of Y-gene loss on spermatogenesis. We use single nuclei RNAseq to identify candidate mechanisms by which Y genes regulate the germ cell transcriptome and reveal an unexpected impact of Y genes on testis supporting cells. Our study represents a paradigm for the complete functional dissection of a mammalian Y chromosome and advances our knowledge of human infertility and Y-chromosome evolution.
Published in
Systematic identification of Y-chromosome gene functions in mouse spermatogenesis
Subrini J, Varsally W, Balsells IB et al. · Science (New York, N.Y.) 2025 · PMID 39847625 · doi:10.1126/science.ads6495
This dataset
Open GSE274095 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274095_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1144950 and SRA study SRP524640. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39847625) ↗
Study design
5 conditions, mostly in duplicate
AZFa_KO ×5 Eif2s3y_KO_P10 ×2 Zfy2 ×2 Ctrl ×2 Zfy1_and_2_DKO ×2 WT_Ctrl_P10 ×1 UTY_KO_P10 ×1 Zfy1 ×1

Supports a between-group comparison across 13 samples.

How we read this: 5 replicated groups read from 16 sample titles; they account for 13 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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