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Transcriptome profiling tumor cells-of-origin from Nf1 and Nf1-Arf null murine model of Neurofibromatosis Type 1

GSE232451 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2024/12/30 Platform GPL24247
Summary
Malignant peripheral nerve sheath tumors (MPNSTs) are the leading cause of premature death for patients with Neurofibromatosis type 1 and no approved targeted therapies are available. Transformation from Nf1-null benign plexiform neurofibromas is driven by the loss of the Cdkn2a (Arf) locus. Here, genetically engineered mouse models with floxed Nf1 and combined Nf1 and Arf alleles were used. Schwann cell precursor cells (the tumor cell-of-origin) were isolated from the nerve root (dorsal root ganglia) of embryos at day 13.5. These cells, termed DRG/nerve root neurosphere cells (DNSCs) were established, and either control adenoviral infection (GFP) was performed, or adenoviral Cre-GFP infection used to conditionally ablate Nf1 or Nf1-Arf. mRNA sequencing was performed using Nf1 (control, GFP infected or “GFP”), Nf1 (Cre-GFP infected or “CRE”), or Nf1-Arf (Cre-GFP infected or “ARF”) to evaluate the effects on the transcriptome.
Published in
DLK1 Distinguishes Subsets of NF1-Associated Malignant Peripheral Nerve Sheath Tumors with Divergent Molecular Signatures
Mitchell DK, Brewster K, Makri SC et al. · Clinical cancer research : an official journal of the American Association for Cancer Research 2025 · PMID 40063513 · doi:10.1158/1078-0432.CCR-24-3029
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Direct links to NCBI, no account and no request form: the whole study as GSE232451_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA972000 and SRA study SRP437460. Searching any of these in the dataset finder brings you back here.

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