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Innate immune memory after brain injury drives inflammatory cardiac dysfunction II

GSE232550 Homo sapiens (human) RNA-seq (gene expression) 20 samples Submitted 2024/07/22 Platform GPL30882
At a glance

RNA-seq (gene expression) · human · 20 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
20 samples: 13 × heart sample, IS patient vs 7 × heart sample, control patientGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Innate immune memory after brain injury drives inflammatory cardiac dysfunctionCell · 2024
Summary (written by the submitters)
Besides the local inflammatory immune response in the brain, stroke also alters systemic immunity. Within the acute and sub-acute phase, the systemic immune response to stroke has been described in detail over last decades. The long-term systemic immunological consequences after stroke are however still elusive. Therefore, a better understanding of these long-lasting chronic effects of stroke on systemic immunity and its impact on remote organ function and secondary comorbidities is needed.
Read the full summary ↓
Here, we investigated the long-term consequences of stroke on the heart. By performing serial cardiac ultrasound imaging in mice, we found that stroke leads to chronic cardiac dysfunction, which was associated with increased cardiac fibrosis. We found that this phenomenon was driven by an increased infiltration of bone marrow-derived monocytes into the heart after stroke, which results in increased expression of metalloproteinase-9 (Mmp9) by cardiac monocytes/macrophages. To further investigate the translational relevance of this post-stroke cardiac phenotype, we analyzed heart samples from patients who died due to stroke and control patients and performed bulk mRNA sequencing.
Published in
Innate immune memory after brain injury drives inflammatory cardiac dysfunction
Simats A, Zhang S, Messerer D et al. · Cell 2024 · PMID 39043180 · doi:10.1016/j.cell.2024.06.028
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE232550_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA972710 and SRA study SRP437730. Searching any of these in the dataset finder brings you back here.

Study design
13 × heart sample, IS patient vs 7 × heart sample, control patient

Supports a between-group comparison across 20 samples.

How we read this: 2 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.