← BioTransfer GEO Dataset Finder
GEO series

The primitive endoderm supports lineage plasticity to enable regulative development [RNA-seq]

GSE232921 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/06/25 Platform GPL30172
Summary
Mammalian blastocyst formation involves the sequential specification of trophectoderm and the differentiation of inner cell mass into either epiblast or primitive endoderm. During this time, the embryo maintains a window of plasticity and able to redirect its cellular fate when challenged experimentally. In this context, we found that the primitive endoderm alone was capable of regenerating a complete blastocyst and continue normal postimplantation development. We identify an in vitro population similar to the early primitive endoderm in vivo that exhibits plasticity, forms three dimensional embryoid structures and exhibits multilineage competence in chimera assays. Here, we find OCT4 as the main player in collaborating with endodermal transcription factors to maintain pluripotent enhancers in a state that is primed for activation mediated by JAK/STAT signalling. Our observations support the notion that transcription factor persistence underlies plasticity in regulative development and highlights the importance of primitive endoderm in perturbed development.
Published in
The primitive endoderm supports lineage plasticity to enable regulative development
Linneberg-Agerholm M, Sell AC, Redó-Riveiro A et al. · Cell 2024 · PMID 38917790 · doi:10.1016/j.cell.2024.05.051
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE232921_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA974427 and SRA study SRP438651. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 8 more — browse all 8 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.