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Transcriptome analysis of Endothelin-3 or Neurotensin treated MuSCs

GSE234590 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/07/25 Platform GPL19057
Summary
GPCRs have emerged as crucial regulators of muscle stem cell (MuSC) quiescence, yet the specific repertoire of GPCRs in quiescent MuSCs and the underlying regulatory network remain largely elusive. By employing pharmacological and inducible-genetic methods, we have identified two niche-derived GPCR ligands, endothelin-3 (ET-3) and neurotensin (NT), which bind to EDNRB and NTSR2 receptors, respectively, reinforcing the maintenance of MuSC quiescence. To comprehensively unravel the molecular mechanisms underlying the inhibitory effects of ET-3 and NT on MuSC activation, we conducted RNA sequencing (RNA-seq) analysis on FACS-purified MuSCs that underwent a 5-day in vitro treatment with ET-3 or NT
Published in
RhoA-mediated G(12)-G(13) signaling maintains muscle stem cell quiescence and prevents stem cell loss
Peng Y, Du J, Li R et al. · Cell discovery 2024 · PMID 39009565 · doi:10.1038/s41421-024-00696-7
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Also filed as BioProject PRJNA982099 and SRA study SRP442297. Searching any of these in the dataset finder brings you back here.

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