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Single cell level characterization of injury-induced immune changes in 10-week-old and 95-week-old C57BL/6 knee joints

GSE236843 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/07/05 Platform GPL19057
Summary
Post-traumatic Osteoarthritis (PTOA) occurs in about half of individuals who sustained articular injuries including tibial plateau fracture, meniscus tear, or an anterior cruciate ligament (ACL) rupture. Damage-associated molecular pattern (DAMP) proteins are typically secreted from injured or dying cells of the synovial joint into the surrounding environment immediately following injury which in turn recruit leukocytes to mediate debris clean-up and repair. Prior bulk RNA-seq experiments exploring organismal age-related differences following ACL rupture found an increased expression of inflammatory-response related genes in 95-week-old mice relative to 10-week-old mice. Using single-cell RNA sequencing technology we temporally explored differences in immune cell infiltration in the synovial joint in 10-week-old and 95-week-old C57BL/6 mice.
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Direct links to NCBI, no account and no request form: the whole study as GSE236843_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA992491 and SRA study SRP448039. Searching any of these in the dataset finder brings you back here.

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