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Next Generation Sequencing Facilitates Quantitative Analysis of Bone Marrow-Derived Macrophages Transcriptomes for Acly-E14 siRNA effects

GSE240188 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/11/06 Platform GPL24247
Summary
Purpose: The purpose of this study is to detect activated or silenced genes during bone marrow derived macrophages (BMDMs) transfected with control siRNA or Acly-E14 siRNA. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. BMDMs were transfected with control siRNA or Acly-E14 siRNA. 48 hour later, they were stimulated with LPS (100ng/ml) for 4 hours, of which RNA profiles were generated by deep sequencing, using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation between BMDMs transfected with the indicated siRNAs.
Published in
RBM25 is required to restrain inflammation via ACLY RNA splicing-dependent metabolism rewiring
Zhang Y, Gao Y, Wang Y et al. · Cellular & molecular immunology 2024 · PMID 39251781 · doi:10.1038/s41423-024-01212-3
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Also filed as BioProject PRJNA1002772 and SRA study SRP453603. Searching any of these in the dataset finder brings you back here.

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