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Murine mesenchymal stem cells undergo molecular changes after a single passage in culture

GSE240514 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/06/05 Platform GPL23479
Summary
The properties of MSCs can be affected by long term culture, therefore casting doubt over the ability of late passage MSCs to accurately recapitulate their biology. Additionally, assumption within the scientific community is often made that early passage MSCs are still representative of the primary MSC population, however, little research has been done to support this. We compared the transcriptomic profiles of murine MSCs freshly isolated from the long bones to MSCs that had been expanded in culture for 10 days. Interestingly, we identified that a single passage in culture induced extensive alterations to MSC molecular signatures associated with cell cycling, differentiation and immune response.
Published in
Murine bone-derived mesenchymal stem cells undergo molecular changes after a single passage in culture
Hughes AM, Kuek V, Oommen J et al. · Scientific reports 2024 · PMID 38811646 · doi:10.1038/s41598-024-63009-8
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Also filed as BioProject PRJNA1003968 and SRA study SRP454357. Searching any of these in the dataset finder brings you back here.

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