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Toxicicty of ruxolitinib in normal mice

GSE240866 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/05/08 Platform GPL24247
Summary
Background and methods: Ruxolitinib (RUX), a Jak1/2 inhibitor, has been reported to attenuate murine bone marrow failure recently. Its potential toxocicty of anemia and thrombocytopenia in human remains a concern. We tested the toxicity of ruxolitinib on hematopoiesis in normal mice by feeding the mice with RUX chow for 3 months. Bone marrow Lin-CD117+ cells were sorted from treated or untreated mice. RNA-Seq and analysis was performed using SMART-Seq mRNA LP Kit (Takara) and the Illumina Novaseq6000, according to the Institute's protocols. Results: Ruxolitinib reduced RBC and lymphocytes, but did not affect NEU and PLT in normal mice. RNA sequencing demonstrated that HSPCs from RUX-treated and untreated mice had overlapped transcriptome distribution in multiple dimentional scalling plot, indicating overall similarity at molecular level. Conclusion: Our results demonstrate that ruxolitinib has minimal toxicity on hematopoiesis in normal mice.
Published in
Efficacy of combined low-dose ruxolitinib and cyclosporine in murine immune bone marrow failure
Feng X, Manley AL, Wu Z et al. · Haematologica 2024 · PMID 38152039 · doi:10.3324/haematol.2023.284358
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Also filed as BioProject PRJNA1005604 and SRA study SRP455174. Searching any of these in the dataset finder brings you back here.

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