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Gene expression profile at the single cell level of CD45+ CD11b+ cells isolated from the brain and bone marrow of mice either untreated or conditioned and transplanted with total bone marrow.

GSE241877 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/13 Platform GPL24247
Summary
We used single-cell RNA sequencing (scRNA-seq) to analyze the diversity of bone marrow-derived CD45+CD11b+ microglia-like cells (MGLCs) engrafted in the brain of recipient mice that were conditioned using Busulfan and PLX3397 and transplanted with total bone marrow. We compared the gene expression of MGLCs to that of developmentally-derived CD45+ CD11b+ microglia/myeloid cells isolated from the brain of recipient mice (host microglia) and untreated mice (naive microglia). We also compared the gene expression of MGLCs to that of transplant-derived CD45+ CD11b+ cells engrafted in the bone marrow (abbreviated as BM-CD11b+)
Published in
CNS-wide repopulation by hematopoietic-derived microglia-like cells corrects progranulin deficiency in mice
Colella P, Sayana R, Suarez-Nieto MV et al. · Nature communications 2024 · PMID 38969669 · doi:10.1038/s41467-024-49908-4
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Direct links to NCBI, no account and no request form: the whole study as GSE241877_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1010649 and SRA study SRP457457. Searching any of these in the dataset finder brings you back here.

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