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Mouse models to investigate in situ cell fate decisions induced by TP53 and other factors (CUT&RUN)

GSE243999 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 23 samples 2024/06/28 GPL30172
Summary
We developed triple-FLAG TRP53 knock-in mice for TRP53 pull-down and two TRP53 response reporter mice, knocking tdTomato or GFP into the Puma/Bbc3 or p21 gene loci, respectively. By crossing the reporter mice onto a TRP53-deficient background, we could show that these reporters reliably inform on TRP53-dependent and TRP53-independent initiation of the apoptotic as well as the cell cycle arrest/senescence programs in vitro and even in vivo.
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