← BioTransfer GEO Dataset Finder
GEO series

Csk restrains BCR-mediated ROS production and contributes to germinal center selection and affinity maturation

GSE244802 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2024/05/06 Platform GPL18480
Summary
Compared with naïve B cells, the B cell receptor (BCR) signal in germinal center (GC) B cells is attenuated; however, the significance of this signaling attenuation has not been well defined. Here, to investigate the role of attenuation of BCR signaling, we employed a Csk mutant mouse model in which Csk-deficiency in GC B cells resulted in augmentation of net BCR signaling with no apparent effect on antigen presentation. We found that Csk is required for GC maintenance and efficient antibody affinity maturation. Mechanistically, ROS-induced apoptosis was exacerbated concomitantly with mitochondrial dysfunction in Csk-deficient GC B cells. Hence, our data suggest that attenuation of the BCR signal restrains hyper-ROS production, thereby protecting GC B cells from apoptosis and contributing to efficient affinity maturation.
Published in
Csk restrains BCR-mediated ROS production and contributes to germinal center selection and affinity maturation
Inoue T, Matsumoto Y, Kawai C et al. · The Journal of experimental medicine 2024 · PMID 38753246 · doi:10.1084/jem.20231996
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE244802_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1023691 and SRA study SRP464409. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 5 more — browse all 5 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.