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Single Cell RNAseq REX-1 RFP mESC 2019

GSE245532 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2024/10/16 Platform GPL24247
Summary
This experiment examines how increasing stress (sorbitol) can override normal stemness (NS) conditions, which LIF maintains, causing lineage imbalance and otherwise affecting cells in the early embryo. It also compares NS to normal differentiation (ND) (without LIF). D3 are from 8 blastocysts of 129/Sv+/+ strain mice germ line competent 1. No sex is given as these may actually be a product of different sexes of the 8 blastocysts. The D3 cells were obtained for ATCC (American Type Culture Collection). 1. Doetschman TC, Eistetter H, Katz M, Schmidt W, Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 1985;87:27-45. Rex1 promoter- Red fluorescence protein (RFP) transgenic viable reporter ESC were created and validated previously2. 2. Li Q, Gomez-Lopez N, Drewlo S, et al. Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells. Stem Cells Dev 2016;25:320-8.
Published in
Novel kinetic and developmental transcriptomic pan-stress responses by embryonic stem cells
Singh A, Ruden XL, Tang W et al. · Scientific reports 2025 · PMID 40594963 · doi:10.1038/s41598-025-06628-z
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Direct links to NCBI, no account and no request form: the whole study as GSE245532_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1028949 and SRA study SRP466713. Searching any of these in the dataset finder brings you back here.

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