GEO series

TertHigh Cells: Key Players in Salivary Gland Homeostasis and Regeneration after Radiation Therapy in Adult Mice

GSE247866 Mus musculus (mouse) RNA-seq (gene expression) 14 samples Submitted 2026/01/01 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 14 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 14 samples: 4 × Tert+ cell, RT, vs 4 × Tert- cell, RT, vs 3 × Tert+ cell, no RT, vs 3 × Tert- cell, no RT,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

To gain insight into the mechanisms by which TertHigh populations regulate cell survival post radiation treatment, we conducted RNA-Seq analysis of sorted TertHigh and TertLow SMG cells treated with or without RT. In this experiment, 2-month-old TertCreERT2/+Rosa26LSL-tdTomato/+ mice were treated with tamoxifen, followed by RT after a 7-day interval. Subsequently, the SMGs were harvested and dissociated 36 hours after RT treatment. We isolated and separately sorted 10,000 TertHigh and TertLow cells for RNA sequencing.

This dataset

Open GSE247866 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE247866_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1040839 and SRA study SRP472173. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

4 × Tert+ cell, RT, vs 4 × Tert- cell, RT, vs 3 × Tert+ cell, no RT, vs 3 × Tert- cell, no RT,

Supports a between-group comparison across 14 samples.

How we read this: 4 replicated groups read from 14 sample titles; they account for 14 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM7901830 Tert+ cell, no RT, rep1 Tert+ cell, no RT,
GSM7901831 Tert+ cell, no RT, rep2 Tert+ cell, no RT,
GSM7901832 Tert+ cell, no RT, rep3 Tert+ cell, no RT,
GSM7901833 Tert- cell, no RT, rep1 Tert- cell, no RT,
GSM7901834 Tert- cell, no RT, rep2 Tert- cell, no RT,
GSM7901835 Tert- cell, no RT, rep3 Tert- cell, no RT,
GSM7901836 Tert+ cell, RT, rep1 Tert+ cell, RT,
GSM7901837 Tert+ cell, RT, rep2 Tert+ cell, RT,
GSM7901838 Tert+ cell, RT, rep3 Tert+ cell, RT,
GSM7901839 Tert+ cell, RT, rep4 Tert+ cell, RT,
GSM7901840 Tert- cell, RT, rep1 Tert- cell, RT,
GSM7901841 Tert- cell, RT, rep2 Tert- cell, RT,
GSM7901842 Tert- cell, RT, rep3 Tert- cell, RT,
GSM7901843 Tert- cell, RT, rep4 Tert- cell, RT,

Datasets on a similar topic

GSE338640 Vascular function underlies enhanced intestinal regeneration after FLASH radiation 26 samples shares regeneration, radiation
GSE307570 Localized FLASH Radiotherapy Reduces Long-Term Skin and Muscle Damage While Preserving Systemic Homeostasis 20 samples shares homeostasis, therapy
GSE337790 Vascular function underlies enhanced intestinal regeneration after FLASH radiation [bulk RNAseq] 18 samples shares regeneration, radiation
GSE251933 Distinct Mammary Stem Cells Orchestrate the Long-term Homeostasis of Adult Mammary Gland 16 samples shares homeostasis, gland
GSE277593 The effect of dual radiation and antiandrogen therapyt on prostate cancer cell lines [Myc-CaP] 12 samples shares radiation, therapy
GSE299441 A Glycolytic Shunt via the Pentose Phosphate Pathway is a Metabolic Checkpoint for Nervous System Sensory Homeostasis and Axonal Regeneration 12 samples shares regeneration, homeostasis
GSE261367 FLASH proton radiotherapy mitigates inflammatory and fibrotic pathways and preserves cardiac function in a preclinical mouse model of radiation-induced heart disease 11 samples shares radiation, therapy
GSE277443 EMP1 preserves hematopoietic stem cell potential under homeostasis and radiation-induced injury by alleviating ER stress [ER-stress RNA-seq] 10 samples shares homeostasis, radiation

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.