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scRNA-seq analysis of human iPSC derived Hepatocyte organoids (iHOs) [scRNA-seq]

GSE247961 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/05/13 Platform GPL28038
Summary
Liver has abundant regenerative capacity, but hepatocytes cannot be cultured long-term in vitro. Activation of FXR signaling induced iHOs with both hepatocyte traits and proliferative potential. To investigate the transcriptomic analysis of iHOs, we performed bulk RNA-seq analysis of organoids in different passages and single cell RNA-seq analysis of iHOs. Compared to 2D-iPS-Heps and organoids in other culture conditions, iHOs have expression profiles more similar to primary human hepatocytes and human fetal liver cells. Single cell RNA-seq revealed iHOs have a fraction that co-expresses hepatocyte markers and proliferation markers.
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Also filed as BioProject PRJNA1041311 and SRA study SRP472346. Searching any of these in the dataset finder brings you back here.

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