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Ptk2 ablation in a Trp53fl/fl, Ptenfl/fl, Rb1fl/fl mouse brain tumor model

GSE249154 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/10 Platform GPL24247
Summary
Ptk2 was identified as a targetable genetic vulnerability in glioblastoma (GBM) cells. Our objective was to test the effect of Ptk2 deletion on survival and tumor phenotype in a mouse model of GBM. At P0, mice carrying Trp53fl/fl (Trp53tm1Brn, JAX #008462), Ptenfl/fl (Ptentm1Hwu/J, JAX #006440), Rb1fl/fl and R26-LSL-Cas9-GFP (JAX #026175) alleles were subjected to stereotactic injection to deliver lentiviral particles expressing Cre and either Ptk2-targeting or control gRNAs to the NSCs residing in the lateral subventricular zone. This injection initiates a GBM tumor in the mice. At time of sacrifice, tumor tissue was harvested and RNA was extracted for sequencing.
Published in
Fitness Screens Map State-Specific Glioblastoma Stem Cell Vulnerabilities
MacLeod G, Molaei F, Haider S et al. · Cancer research 2024 · PMID 39186687 · doi:10.1158/0008-5472.CAN-23-4024
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Also filed as BioProject PRJNA1047561 and SRA study SRP475331. Searching any of these in the dataset finder brings you back here.

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