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Integrated multi-omics analysis of zinc finger proteins uncovers roles in RNA regulation.

GSE249247 synthetic construct; Homo sapiens RNA-seq (gene expression) 630 samples Submitted 2024/09/20 Platform GPL34014Platform GPL24676
At a glance

RNA-seq (gene expression) · synthetic construct; Homo sapiens · 630 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
synthetic construct; Homo sapiens
Samples
630 samples: 292 distinct titles, 68 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Integrated multi-omics analysis of zinc-finger proteins uncovers roles in RNA regulationMolecular cell · 2024
SuperSeries — this record groups several sub-series.
Summary (written by the submitters)
This SuperSeries is composed of the SubSeries listed below.
Published in
Integrated multi-omics analysis of zinc-finger proteins uncovers roles in RNA regulation
Gosztyla ML, Zhan L, Olson S et al. · Molecular cell 2024 · PMID 39303722 · doi:10.1016/j.molcel.2024.08.010
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE249247_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1048308 and SRA study SRP475585. Searching any of these in the dataset finder brings you back here.

Study design
292 distinct titles, 68 of them repeated
BRCA1 knockdown 0hr-chase ×3 BRCA1 knockdown 2hr-chase ×3 BRCA1 knockdown 4hr-chase ×3 BRCA1 knockdown 8hr-chase ×3 CNOT4 knockdown 0hr-chase ×3 CNOT4 knockdown 2hr-chase ×3 CNOT4 knockdown 4hr-chase ×3 CNOT4 knockdown 8hr-chase ×3 +60 more

Supports a case/control comparison: 120 samples read as cases, 8 as controls.

How we read this: Read from the first 400 of 630 sample titles: 292 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 590 more — browse all 630 samples with per-sample file links →

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