Gene expression profiles under oxidative stress conditions in human HaCaT keratinocytes and the derivative cells expressing shRNA against NRF2, MLL1, or UTX.
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- MLL1 histone methyltransferase and UTX histone demethylase functionally cooperate to regulate the expression of NRF2 in response to ROS-induced oxidative stressFree radical biology & medicine · 2024
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE250127_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1052078 and SRA study SRP477834. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7974261 shControl, Untreated
- GSM7974262 shControl, 100 uM H2O2
- GSM7974263 shControl, 25 ug/mL CeO2 NP
- GSM7974264 shMLL1, Untreated
- GSM7974265 shMLL1, 100 uM H2O2
- GSM7974266 shMLL1, 25 ug/mL CeO2 NP
- GSM7974267 shUTX, Untreated
- GSM7974268 shUTX, 100 uM H2O2
- GSM7974269 shUTX, 25 ug/mL CeO2 NP
- GSM7974270 shNRF2, Untreated
- GSM7974271 shNRF2, 100 uM H2O2
- GSM7974272 shNRF2, 25 ug/mL CeO2 NP
- GSE250128 MLL1 histone methyltransferase and UTX histone demethylase functionally cooperate to regulate the expression of NRF2 in response to ROS-induced oxidative stress 21 samples shares nrf2, mll1, utx, oxidative
- GSE308958 Comparative Analysis of Oral and Skin Keratinocytes Responses to Oxidative Stress 12 samples shares keratinocytes, oxidative
- GSE248548 Effect of IQGAP3 silencing on HaCaT keratinocytes under normal conditions or inflammatory cytokine stimulation 12 samples shares keratinocytes, conditions
- GSE320415 Paeonia delavayi seed oil alleviates UVB-induced inflammation, oxidative stress and photoaging in keratinocytes through inhibition of PI3K/Akt/mTOR and NF-κB signaling pathways 9 samples shares keratinocytes, oxidative
- GSE293361 Genetic Alterations in KEAP1-NRF2 system Induced by Anti-Cancer Treatments Contribute to Cisplatin Resistance in Head and Neck Squamous Cell Carcinoma 42 samples shares nrf2
- GSE301297 Functional redundancy between UTY and UTX in regulating the localization of transcription factors involved in pluripotency [RNA-seq] 40 samples shares utx
- GSE262660 SCD1-dependant activation of GSK3β-NRF2-SLC7A11 pathway regulates lipid metabolism and suppresses ferroptosis in KRAS/STK11/KEAP1 co-mutant lung adenocarcinoma 36 samples shares nrf2
- GSE282741 Menin inhibitor DS-1594b drives differentiation and induces synergistic lethality in combination with Venetoclax in acute myeloid leukemia cells with rearranged MLL1 and NPM1 mutations 36 samples shares mll1
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.