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Expression data from cultured spermatogonia established from Cdkn1c conditional knockout mouse testes

GSE253319 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/01/30 Platform GPL30172
Summary
Mice lacking Cdkn1c (p57) in spermatogonia were found to be congenitally infertile due to spermatogonia depletion. Transplantation experiments showed that Cdkn1c deficiency compromised spermatogonial stem cells (SSC) activity. SSCs undergo self-renewal division to sustain spermatogenesis, and it is possible to derive germline stem (GS) cell cultures by supplementing GDNF and FGF2. We examined the influence of Cdkn1c depletion on gene expression of GS cells. In this dataset, we include the expression data obtained from cultured spermatogonia (GS cells) derived from Cdkn1c(p57) floxed/floxed mice. Cdkn1c(p57) floxed/floxed GS cells were treated with adenovirus cre recombinase. Each group contains 3 biological replicates.
Published in
Cdkn1c orchestrates a molecular network that regulates euploidy of male mouse germline stem cells
Kanatsu-Shinohara M, Yamamoto T, Liu T et al. · Development (Cambridge, England) 2025 · PMID 39850012 · doi:10.1242/dev.204286
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Direct links to NCBI, no account and no request form: the whole study as GSE253319_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1065281 and SRA study SRP483948. Searching any of these in the dataset finder brings you back here.

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