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Adenoviral vector transduced in vitro differentiated C57bl6 BMDM cells

GSE253342 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/04/21 Platform GPL21103
Summary
Total mRNA was extracted at 48 hour post transduction using Qiagen RNeasy Kits following the manufacture’s protocol.1 ug ofRNAwas used for cDNA library construction and bulk RNA sequencing were performed by Novogene (Sacramento CA). RNA-seq raw data were analysised using Partek Flow. Briefly, sequencing reads were aligned to the mouse or human genome using Spliced Transcripts Alignment to a Reference (STAR 2.5.3a). Aligned reads were then quantified to transcriptome (mm10; Ensembl Transcripts release 100), and normalized (Median Ratio normalization). Differentiated gene expression analysis (DESeq2) were performed to generate raw counts
Published in
CAR macrophages with built-In CD47 blocker combat tumor antigen heterogeneity and activate T cells via cross-presentation
Chen S, Wang Y, Dang J et al. · Nature communications 2025 · PMID 40307254 · doi:10.1038/s41467-025-59326-9
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Direct links to NCBI, no account and no request form: the whole study as GSE253342_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1065357 and SRA study SRP483996. Searching any of these in the dataset finder brings you back here.

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