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Tumor microenvironment landscapes supporting EGFR-mutant NSCLC are modulated at the single cell interaction level by Unesbulin treatment

GSE253658 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2024/04/03 Platform GPL19057
Summary
Lung cancer is the leading cause of cancer deaths. Lethal pulmonary adenocarcinomas (ADCs) present with frequent mutations in the Epidermal Growth Factor Receptor (EGFR). Genetically-engineered murine models of lung cancer expedited comprehension of the molecular mechanisms driving tumorigenesis and drug response. Here, we systematically analyzed the evolution of tumor heterogeneity in the context of dynamic interactions occurring with the intermingled tumor microenvironment by high resolution transcriptomics. Our effort identified vulnerable tumor-specific epithelial cells, as well as their cross-talk with niche components (endothelial cells, fibroblasts and tumor-infiltrating immune cells), whose symbiotic interface shapes tumor aggressiveness and is almost completely abolished by treatment with Unesbulin, a tubulin binding agent that reduces BMI-1 activity. Simultaneous MRI analysis demonstrated decreased tumor growth, setting the stage for future investigations into the potential of novel therapeutic strategies for EGFR-mutant ADCs.
Published in
Tumor Microenvironment Landscapes Supporting EGFR-mutant NSCLC Are Modulated at the Single-cell Interaction Level by Unesbulin Treatment
Maroni G, Krishnan I, Alfieri R et al. · Cancer research communications 2024 · PMID 38546390 · doi:10.1158/2767-9764.CRC-23-0161
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Direct links to NCBI, no account and no request form: the whole study as GSE253658_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1066727 and SRA study SRP484698. Searching any of these in the dataset finder brings you back here.

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