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Psychological Stress-Induced Systemic Corticosterone Directly Sabotages Intestinal Stem Cells Activity

GSE253827 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/03/14 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Ctrl vs 3 × Vehicle vs 3 × CORTGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Psychological stress-induced systemic corticosterone directly sabotages intestinal stem cells and exacerbates colitisCell discovery · 2025
Summary (written by the submitters)
Stress exerts profound impacts on the gastrointestinal tract, yet its effects on intestinal stem cells (ISCs) have not been fully explored. Here, we unveiled a notable reduction in both the quantity and proliferative capacity of ISCs under chronic stress condition, which was mediated by elevated corticosterone levels resulting from the activation of the hypothalamic-pituitary-adrenal (HPA) axis.
Read the full summary ↓
Mechanistically, corticosterone directly impairs ISCs by interacting with its receptor, nuclear receptor subfamily 3 group c member 1 (NR3C1), which promoted FKBP prolyl isomerase 5 (FKBP5) expression to negatively regulate the AKT activation by mediating the dephosphorylation at the Ser473, thus enhancing the nuclear translocation of the forkhead box O (FoxO) to inhibit the proliferative activity. Collectively, these findings provide a novel mechanistic insight into the deleterious effects of psychological stress on gut, and underscore corticosterone as a key mediator linking stress to ISCs dysfunction.
Published in
Psychological stress-induced systemic corticosterone directly sabotages intestinal stem cells and exacerbates colitis
Sheng X, Jin L, Yao Z et al. · Cell discovery 2025 · PMID 40360481 · doi:10.1038/s41421-025-00796-y
This dataset
Open GSE253827 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE253827_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1067346 and SRA study SRP485049. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40360481) ↗
Study design
3 × Ctrl vs 3 × Vehicle vs 3 × CORT

Supports a between-group comparison across 9 samples.

How we read this: 3 replicated groups read from 12 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.