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RNA-Seq data of mouse retina, retinal pigment epithelium and cones

GSE254669 Mus musculus (mouse) RNA-seq (gene expression) 254 samples Submitted 2024/07/05 Platform GPL21493Platform GPL24247Platform GPL17021
At a glance

RNA-seq (gene expression) · mouse · 254 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
254 samples: 28 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Cone photoreceptor differentiation regulated by thyroid hormone transporter MCT8 in the retinal pigment epitheliumProceedings of the National Academy of Sciences of the United States of America · 2024
Summary (written by the submitters)
The differentiation of cone photoreceptors, which mediate daylight vision and color vision, depends critically upon thyroid hormone.However, the route of access of blood-borne thyroid hormone to cones is undefined because cones reside behind the blood-retina barrier.
Read the full summary ↓
This study uses genetic manipulation of a membrane transporter for thyroid hormone (Mct8) in mouse models to show a role for the retinal pigment epithelium (RPE), which forms the outer blood-retina barrier, in the control of cone differentiation.The results suggest a paracrine-like mechanism promotes thyroid hormone-mediated cone differentiation. The findings suggest that in addition to the transport of essential solutes and support of photoreceptor homeostasis, the RPE controls hormonal signaling required for cone differentiation.
Published in
Cone photoreceptor differentiation regulated by thyroid hormone transporter MCT8 in the retinal pigment epithelium
Liu Y, Ng L, Liu H et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 39018199 · doi:10.1073/pnas.2402560121
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE254669_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1071483 and SRA study SRP487055. Searching any of these in the dataset finder brings you back here.

Study design
28 conditions, mostly in duplicate
2m_cone_ctrl_inf ×44 2m_cone_ctrl_sup ×43 2m_cone_KO_inf ×43 2m_cone_KO_sup ×42 RPE_inf ×5 RPE_sup ×5 P14_female_mct8_het_cre_cko_retina_sup_D ×5 P14_female_mct8_het_cre_cko_retina_inf_D ×5 +26 more

Supports a between-group comparison across 248 samples.

How we read this: 28 replicated groups read from 254 sample titles; they account for 248 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 214 more — browse all 254 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.