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Ex vivo imaging reveals the spatiotemporal control of ovulation

GSE255274 Mus musculus (mouse) RNA-seq (gene expression) 28 samples Submitted 2024/07/08 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 28 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
28 samples: 10 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
To investigate the transcriptomic profile of in vivo and ex vivo ovulation systems
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE255274_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1074329 and SRA study SRP488754. Searching any of these in the dataset finder brings you back here.

Study design
10 conditions, mostly in triplicate
In vitro setup, 0hr post-hCG administration, ×3 In vitro setup, 3hr post-hCG administration, ×3 In vivo setup, 6hr post-hCG administration, ×3 In vitro setup, 6hr post-hCG administration, ×3 In vivo setup, 9hr post-hCG administration, ×3 In vitro setup, 9hr post-hCG administration, ×3 In vivo setup, 12hr post-hCG administration, ×3 In vitro setup, 12hr post-hCG administration, ×3 +2 more

Supports a between-group comparison across 28 samples.

How we read this: 10 replicated groups read from 28 sample titles; they account for 28 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.