← BioTransfer GEO Dataset Finder
GEO series

Single-cell gene expression profile of cultured mouse islet cells

GSE255447 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/07/29 Platform GPL28457
Summary
Dysregulated α-cell function contributes to the onset and progression of diabetes. Here, we report that the treatment with imeglimin, an anti-diabetes drug, prevents glucagon release and promotes α-to-β cell transdifferentiation through direct action on α-cells. Mechanistically, imeglimin reduces Gsα expression to attenuate EPAC2-mediated glucagon secretion induced by glucose or GIP in an insulin-independent fashion. MafB expression is downregulated by imeglimin to make α-cell transdifferentiate to β-cell. Imeglimin also upregulates CHOP expression, which partly contributes to the reduction in both Gsα and MafB expression to reduce glucagon secretion and induce α-cell dedifferentiation, without alteration of protein translation. Those pleiotropic effects of imeglimin on glucagon secretion and α-cell identity can be recapitulated by mouse models of diabetes in vivo. These data implicate the imeglimin-mediated regulation of α-cell plasticity as a novel component in diabetes therapy to correct islet functions.
Published in
Imeglimin suppresses glucagon secretion and induces a loss of α cell identity
Tsuno T, Li J, Nishiyama K et al. · Cell reports. Medicine 2025 · PMID 40713970 · doi:10.1016/j.xcrm.2025.102254
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE255447_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1075053 and SRA study SRP489114. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.