GEO series

Gene expression changes induced by Elp1 deficiency during early myeloid differentiation [elp1]

GSE255735 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/07/18 Platform GPL24247

Summary

The replacement of microglia with myeloid cells derived from transplanted bone marrow represents a promising cell therapeutic concept for different neurological disorders. Previous studies have primarily been based on freshly harvested grafts, permitting only limited control over cells prior to transplantation. Here we demonstrate the ability of in vitro expanded hematopoietic stem and progenitor cells (cHSPCs) to engraft in the myeloid niche of the brain, and effectively replace endogenous microglia both in vivo and in vitro. Utilizing genetic editing of cHSPCs, we identify elongator acetyltransferase complex subunit 1 (Elp1; also known as Ikbkap; encoding for the protein IKAP) as an essential gene for the generation of cHSPC-derived myeloid cells (HDMCs). In accordance with its role as a subunit of the Elongator complex, loss of Elp1 in early-stage HDMCs led to upregulation of a gene module associated with the unfolded protein response.

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Direct links to NCBI, no account and no request form: the whole study as GSE255735_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

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Also filed as BioProject PRJNA1076248 and SRA study SRP489625. Searching any of these in the dataset finder brings you back here.

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