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Sex differences in Non-Alcoholic Steatohepatitis (NASH) in mice evoked by a fructose-palmitate-cholesterol (FPC)-enriched diet [single_nucleus]

GSE256503 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/09/24 Platform GPL24247
Summary
To study sex differences in the response to NASH in mice, single nucleus RNA sequencing of livers was performed. Single nucleus RNAseq demonstrated enrichment of differentially-expressed genes particularly in hepatocytes and stellate cells in females versus males fed FPC-NASH. Multiple regulons (transcription factors and target genes) implicated in lipid and other metabolic processes and hormone signaling (hepatocytes) and cell-cell communication and cell adhesion (stellate cells) were noted, which were enriched in female versus male FPC-NASH diet. Bulk liver RNAseq and RNAseq of isolated hepatocytes and hepatic stellate cells were analyzed as validation experiments.
Published in
Sex differences in murine MASH induced by a fructose-palmitate-cholesterol-enriched diet
Arivazhagan L, Delbare S, Wilson RA et al. · JHEP reports : innovation in hepatology 2025 · PMID 39911943 · doi:10.1016/j.jhepr.2024.101222
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Also filed as BioProject PRJNA1079802 and SRA study SRP491428. Searching any of these in the dataset finder brings you back here.

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