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iFlpMosaic enable the multispectral barcoding and high-throughput comparative analysis of mutant and wildtype cells.

GSE257723 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/09/03 Platform GPL30172
Summary
In order to understand gene function, cells mutated for a gene need to be compared with normal cells. In most biomedical studies this comparative analysis is carried out with cells present in different animals and therefore not experiencing the same microenvironment or epigenetic changes. Here we present a large set of new genetic tools and mouse lines, that enable the Flp recombinase-dependent ratiometric induction and single cell clonal tracking of multiple fluorescently labelled normal and Cre-mutant cells, from distinct or the same progenitor cells. The labelled cells can be profiled in situ by multispectral imaging, or by FACS and scRNA-seq. With these new tools, normal and mutant cells can be ratiometrically induced and multispectrally barcoded within the same temporal window and tissue microenvironment. This enables a better understanding of how induced genetic mutations affect the biology of single cells with higher accuracy and reliability during tissue development, homeostasis, or disease.
Published in
iFlpMosaics enable the multispectral barcoding and high-throughput comparative analysis of mutant and wild-type cells
Garcia-Gonzalez I, Gambera S, Rocha SF et al. · Nature methods 2025 · PMID 39672980 · doi:10.1038/s41592-024-02534-w
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Direct links to NCBI, no account and no request form: the whole study as GSE257723_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1080217 and SRA study SRP491612. Searching any of these in the dataset finder brings you back here.

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