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Transcriptome of E9.5 WT and Smyd1-KO mouse hearts

GSE260692 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/04/18 Platform GPL17021
Summary
Purpose: The goal of this study is to identify the differential cardiac transcriptome profiling between WT and Smyd1 null (Smyd1-KO) hearts at E9.5 using RNA-seq. Methods: mRNA profiles of E9.5 WT and Smyd1-KO mouse hearts were generated by deep sequencing, n=3 for each genotype, using Illumina HiSeq2500. The sequence reads were aligned to the mm10 reference genome using STAR via the bcbio-nextgen RNA-sequencing pipeline. Differential gene expression was determined by DEseq2. Results: 1756 genes were differentially expressed between WT and Smyd1-KO hearts [adjusted P value <0.05, |log2(Fold Change)| > 0.5], with 1130 upregulated and 626 downregulated in E9.5 Smyd1-KO hearts.
Published in
CHD4 and SMYD1 repress common transcriptional programs in the developing heart
Shi W, Wasson LK, Dorr KM et al. · Development (Cambridge, England) 2024 · PMID 38619323 · doi:10.1242/dev.202505
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Also filed as BioProject PRJNA1082680 and SRA study SRP492838. Searching any of these in the dataset finder brings you back here.

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