GEO series

Murine anaphylaxis to food requires cysteinyl leukotriene-mediated absorption of allergens in the gut

GSE261022 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/08/13 Platform GPL16417

At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 18 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Cysteinyl leukotrienes stimulate gut absorption of food allergens to promote anaphylaxis in miceScience (New York, N.Y.) · 2025

Summary (written by the submitters)

Food-specific IgE triggers life-threatening anaphylaxis; however, some people with food-specific IgE are asymptomatic upon allergen consumption. Using murine food allergy models, we discovered an unexpected pathway regulating the ability of food allergens to trigger anaphylaxis. C57BL/6 mice are uniquely resistant to anaphylaxis when challenged orally; we show that their gut barrier was impermeable to food allergens relative to anaphylaxis-susceptible strains, even prior to allergic sensitization.

Read the full summary ↓

In a forward genetic screen, oral anaphylaxis resistance correlated with Dipeptidase1 (Dpep1) allelic variants. DPEP1 is expressed in intestinal epithelium and catabolizes leukotriene D4. Blocking DPEP1 with cilastatin enhanced allergen absorption in resistant mice. Conversely, pre-treatment of susceptible mice with a leukotriene synthesis inhibitor, zileuton, abrogated allergen absorption and subsequent oral anaphylaxis. Inhibiting leukotrienes may be a novel treatment for food allergy.

Published in

Cysteinyl leukotrienes stimulate gut absorption of food allergens to promote anaphylaxis in mice

Hoyt LR, Liu E, Olson EC et al. · Science (New York, N.Y.) 2025 · PMID 40773557 · doi:10.1126/science.adp0240

This dataset

Open GSE261022 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE261022_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1084834 and SRA study SRP493698. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40773557) ↗

Study design

A cohort of 18 individual samples

The grouping that defines any comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Sample titles run SCE33 … SCE50 and carry no group information.

Samples in this study

GSM8132742 SCE33
GSM8132743 SCE34
GSM8132744 SCE35
GSM8132745 SCE36
GSM8132746 SCE37
GSM8132747 SCE38
GSM8132748 SCE39
GSM8132749 SCE40
GSM8132750 SCE41
GSM8132751 SCE42
GSM8132752 SCE43
GSM8132753 SCE44
GSM8132754 SCE45
GSM8132755 SCE46
GSM8132756 SCE47
GSM8132757 SCE48
GSM8132758 SCE49
GSM8132759 SCE50

Datasets on a similar topic

GSE285568 Murine anaphylaxis to food requires cysteinyl leukotriene-mediated absorption of allergens in the gut. 8 samples shares leukotriene-mediated, anaphylaxis, absorption, cysteinyl, allergens, requires, food
GSE279083 Lysine acetylation of aquaporin-3 promotes water reabsorption but is not essential for urine concentration 28 samples shares absorption
GSE304800 Bile acids regulate lipid metabolism through selective actions on fatty acid absorption 16 samples shares absorption
GSE317653 Liver-specific loss of the transcriptional coregulator ARGLU1 protects against diet-induced obesity in mice through decreased lipid absorption. 16 samples shares absorption
GSE296733 Elevated Ozone Activates Maternal Immunity to Drive Offspring IgG-Mediated Anaphylaxis through IgG Glycosylation Reprogramming 12 samples shares anaphylaxis
GSE317716 Influence of gut microbiota on oral drug absorption and metabolism 11 samples shares absorption
GSE295380 Pla2g12b is critical for intestinal lipid absorption and C129Y is a dominant negative mutation 9 samples shares absorption
GSE272386 Impaired nutrient absorption and alterations in gut microbiome contribute to reduced postnatal growth in Zeb2+/- mice 8 samples shares absorption

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.