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Elevated Ozone Activates Maternal Immunity to Drive Offspring IgG-Mediated Anaphylaxis through IgG Glycosylation Reprogramming

GSE296733 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/06/26 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × mouse lung (PND14), control, vs 3 × mouse lung (PND14), ozone, vs 3 × mouse lung (PND56), control, vs 3 × mouse lung (PND56), ozone,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
The global burden of allergic diseases has reached alarming proportions and environmental factors exert a strong influence. Ozone (O3), with escalating pollution levels, has emerged as a critical and pressing environmental challenge worldwide and elicits pervasive oxidative stress among population. Epidemiological studies have linked prenatal oxidative stress to offspring allergy susceptibility, while existing evidence lacks causality.To address these gaps, correlation between hematological abnormalities in the retrospective mother-infant cohort and the daily O3 level was analyzed.
Read the full summary ↓
Gestational MIA was induced via O3 exposure at environmentally relevant concentrations from embryonic day 9.5 (E9.5) onward, allowing for an in-depth examination of how neonatal IgG glycosylation is programmed.Using transcriptomic sequencing technologies, we further analyzed the subsequent effects on inflammatory pathways. Utilizing established allergic challenge models, long-term immune consequences in offspring were systematically evaluated, with a specific focus on elucidating the mechanistic role of glycosylated IgG in predisposing MIA progeny to allergic reactions.
This dataset
Open GSE296733 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296733_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1260975 and SRA study SRP584167. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × mouse lung (PND14), control, vs 3 × mouse lung (PND14), ozone, vs 3 × mouse lung (PND56), control, vs 3 × mouse lung (PND56), ozone,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.